Correlation between Hypomethylation of DNA and Expression of Globin Genes in Friend Erythroleukemia Cells
Open Access
- 1 November 1977
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 81 (1), 53-61
- https://doi.org/10.1111/j.1432-1033.1977.tb11926.x
Abstract
This report identifies l-ethionine as an inducer of differentiation in murine erythroleukemia cells. When Friend erythroleukemia cells are grown in the presence of 4 mM l-ethionine, globin mRNA accumulates and in 4–5 days, 25–30% of the cells in the culture contain hemoglobin. Incubation of the cells with bromodeoxyuridine prevents both ethionine-induced accumulation of globin mRNA and erythroid differentiation. At the concentration where l-ethionine acts as an inducer of FL cell differentiation it inhibits methylation of DNA and tRNA in vivo but does not prevent macromolecular synthesis or cell division. To establish whether a link existed between inhibition of a specific methyltransferase and activation of globin synthesis in FL cells, we examined the degree of hypomethylation of DNA and tRNA from FL cells induced to differentiate with dimethylsulfoxide and butyrate. In contrast to the tRNA from ethionine-treated cells, tRNA from cells induced by butyrate or Me2SO cannot be methylated in vitro using homologous enzymes. DNA isolated from cells exposed to any of the three inducers, however, was significantly hypomethylated when compared with DNA from uninduced cells. These data suggest that methylation of DNA may play a role in the regulation of gene expression.This publication has 60 references indexed in Scilit:
- How Are Alkynes Scrambled?Science, 2005
- Building for EarthquakesScience, 2004
- The methylation of tRNABiochimie, 1976
- X inactivation, differentiation, and DNA methylationCytogenetic and Genome Research, 1975
- Metabolic controls in precancerous liver—VI. Studies in vivo and in vitro on feedback regulation of Δ-aminolevulinic acid synthetase in ethionine-fed ratsEuropean Journal of Cancer (1965), 1974
- Mechanism of action of rat liver DNA methylase: II. Interaction with single-stranded methyl-acceptor DNAJournal of Molecular Biology, 1971
- Rare Bases in Animal DNANature, 1970
- Effect of the methionine analogs, ethionine and norleucine, on DNA synthesis in Escherichia coli 15T−Journal of Molecular Biology, 1968
- Synthesis of low molecular weight ribonucleic acid in tumour cellsJournal of Molecular Biology, 1966
- A procedure for the isolation of deoxyribonucleic acid from micro-organismsJournal of Molecular Biology, 1961