A modified triple test-cross analysis to test and allow for inadequate testers

Abstract

When the population under investigation consists of highly inbred lines the full triple test-cross of Kearsey and Jinks (1968) supplemented by the selfed progenies of the population allows unambiguous and independent tests for epistasis and the adequacy of the pure-breeding testers, L₁ and L₂. This can also be achieved by supplementing the simplified triple test-cross of Jinks, Perkins and Breese (1969) with the selfed progenies of the L_1i and L_2i families. If the L₁ and L₂ testers prove to be inadequate due to the presence of common loci, modifications of the analyses are proposed which correct the resulting biases in the genetical components of variation.