The influence of a membrane environment on the structure and stability of a prokaryotic potassium channel, KcsA
- 31 August 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (23), 5199-5204
- https://doi.org/10.1016/j.febslet.2005.08.038
Abstract
The lack of a membrane environment in membrane protein crystals is considered one of the major limiting factors to fully imply X-ray structural data to explain functional properties of ion channels [Gulbis, J.M. and Doyle, D. (2004) Curr. Opin. Struct. Biol. 14, 440–446]. Here, we provide infrared spectroscopic evidence that the structure and stability of the potassium channel KcsA and its chymotryptic derivative 1–125 KcsA reconstituted into native-like membranes differ from those exhibited by these proteins in detergent solution, the latter taken as an approximation of the mixed detergent-protein crystal conditionsKeywords
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