CROSSED IMMUNOELECTROPHORETIC ANALYSIS OF BORDETELLA PERTUSSIS ANTIGENS AND OF CORRESPONDING ANTIBODIES IN HUMAN SERA

Abstract
Antigens (44) were demonstrated in sonicated preparations of B. pertussis (B.p.), using crossed immunoelectrophoresis against rabbit antiserum. No qualitative differences between the 4 strains of the Danish pertussis vaccine were found. In preparations of B.p. culture medium, purified with respect to lymphocytosis promoting factor (LPF) activity, 1 antigen was possibly related to LPF. In human sera antibodies against 5 B.p. antigens were demonstrated by crossed immunoelectrophoresis with intermediate gel. Antibody production was demonstrable in children during the pertussis vaccination period and was most marked after the 2nd vaccination.