IDENTIFICATION OF 3-O-METHYL-ALPHA-METHYLDOPAMINE AS A URINARY METABOLITE OF 3,4-METHYLENEDIOXYAMPHETAMINE IN DOG AND MONKEY

Abstract

3-O-Methyl-.alpha.-methyldopamine was separated by GLC as a metabolite of MDA [3,4-methylene dioxyamphetamine] in the urine of dog and monkey [Macaca mulatta]. The metabolite was identified as its mono- and di-trifluoroacetyl derivatives by comparison of their GLC and GLC-mass spectral properties with those of synthetic compounds. The amount of metabolite increased on hydrolyzing the urine from dosed dogs and monkeys with a preparation containing .beta.-glucuronidase and sulfatase [MDA is a frequently abused drug.].