Induction of macrophage DNA synthesisin vitro by non-immunological inflammatory exudates: Effect of irradiation and thymus or bone marrow cell reconstitution

Abstract

An acute inflammatory exudate possesses mitogenic activity; induces DNA synthesis and proliferation of macrophages in vitro. This activity is reduced if the inflammatory exudate is obtained from irradiated rats (900 R). Transfer of bone marrow syngeneic cells into irradiated rats does not reverse this reduction. The decrease of mitogenic activity is more pronounced. Transfer of thymic syngeneic cells not only restores the mitogenic activity of inflammatory exudate from irradiated rats but increase it. Transfer of both types of cells together fully restores the mitogenic activity of inflammatory exudate. The mitogenic activity of inflammatory exudate could be related to thymic cells. T [thymus-derived] lymphocytes may be involved in non-specific inflammatory reactions.