Tyrosine Hydroxylase Activity in Rat Brain Synaptosomes: Direct Measurement Using High Performance Liquid Chromatography
- 1 April 1982
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 38 (4), 1139-1143
- https://doi.org/10.1111/j.1471-4159.1982.tb05360.x
Abstract
By use of high performance liquid chromatography with electrochemical detection to measure dopamine production, tyrosine hydroxylase (EC 1.14.16.2) activity was measured in rat brain synaptosomes from striatum and forebrain. Normal specific activities 3- to 5-fold higher than previously reported in the literature for radiochemical methods of assay were found. Synaptosomes may contain a significant amount of endogenous substrate for tyrosine hydroxylase, which causes dilution of the added labeled tyrosine and underestimation of the activity of this enzyme when radiochemical methods are used.Keywords
This publication has 12 references indexed in Scilit:
- SYNAPTOSOMAL TYROSINE HYDROXYLATION IN THE RAT BRAIN: COMPARISON OF ACTIVITY FROM HIPPOCAMPUS AND HYPOTHALAMUS WITH ACTIVITY FROM STRIATUMJournal of Neurochemistry, 1978
- DETERMINATION OF CATECHOLAMINES IN RAT BRAIN PARTS BY REVERSE‐PHASE ION‐PAIR LIQUID CHROMATOGRAPHYJournal of Neurochemistry, 1978
- DOPAMINE BIOSYNTHESIS FROM L‐TYROSINE AND L‐PHENYLALANINE IN RAT BRAIN SYNAPTOSOMES: PREFERENTIAL USE OF NEWLY ACCUMULATED PRECURSORS1,2Journal of Neurochemistry, 1977
- Liquid chromatographic analysis of catecholamines routine assay for regional brain mappingLife Sciences, 1976
- Activation by cyclic 3':5'-adenosine monophosphate of tyrosine hydroxylase in the rat brain.Proceedings of the National Academy of Sciences, 1975
- REGULATION OF CATECHOLAMINE SYNTHESIS IN RAT BRAIN SYNAPTOSOMES1Journal of Neurochemistry, 1974
- Tyrosine hydroxylase in rat brain—cofactor requirements, regional and subcellular distributionBiochemical Pharmacology, 1972
- Solubilization and Partial Purification of Tyrosine Hydroxylase from Bovine Adrenal MedullaJournal of Biological Chemistry, 1971
- INCREASE IN RAT BRAIN TYROSINE HYDROXYLASE ACTIVITY PRODUCED BY ELECTROCONVULSIVE SHOCKProceedings of the National Academy of Sciences, 1969
- Some observations upon a new inhibitor of monoamine oxidase in brain tissueBiochemical Pharmacology, 1968