Nuclease protection analysis of ribonucleoprotein complexes: use of the cytotoxic ribonuclease alpha-sarcin to determine the binding sites for Escherichia coli ribosomal proteins L5, L18, and L25 on 5S rRNA.
- 1 January 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (2), 322-326
- https://doi.org/10.1073/pnas.81.2.322
Abstract
A rapid and convenient method was devised to determine the binding sites on RNA. The procedure is an adaptation of one used to map DNA-protein complexes by protection against nuclease digestion. The method uses the cytotoxic RNase .alpha.-sarcin, which hydrolyzes purines in single- and double-stranded regions of RNA. It was authenticated by confirming the binding sites for the E. coli ribosomal proteins L18 and L25 on 5S rRNA and its value was established by identifying the attachment site for protein L5. The procedure should be useful for the analysis of other ribonucleoprotein complexes.This publication has 29 references indexed in Scilit:
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