Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae.
- 1 August 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (15), 4642-4646
- https://doi.org/10.1073/pnas.81.15.4642
Abstract
S. cerevisiae cells were transformed with plasmids containing hybrid genes in which the sequence encoding mature human epidermal growth factor was joined to sequences encoding the leader region (preprosegment) of the precursor of the yeast malting pheromone .alpha.-factor. These cells accurately process the hybrid protein and efficiently secrete authentic biologically active human epidermal growth factor into the medium.This publication has 23 references indexed in Scilit:
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