Solubilization and Characterization of a [3H]Hemicholinium-3 Binding Site in Rat Brain
- 1 June 1988
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 50 (6), 1759-1764
- https://doi.org/10.1111/j.1471-4159.1988.tb02475.x
Abstract
A sodium-dependent high-affinity [3H]hemicholinium-3 ([3H]HCh-3) binding site was solubilized from rat striatal synaptic plasma membranes by 0.2% deoxycholate. Deoxycholate solubilization of the [3H]HCh-3 binding site was dependent upon both detergent concentration and ionic strength of the solubilization medium. Specific [3H]HCh-3 binding of the solubilized preparation was both sodium- and chloride-dependent and saturable, exhibiting an affinity of 14.2 nM and a capacity (Bmax) of 695 fmol/mg protein. Choline and other analogs inhibited specific [3H]HCh-3 binding to the solubilized preparation in a concentration-dependent manner with the similar rank order of potency observed in crude synpatic membranes. Treatments known to disrupt both protein and lipid moieties resulted in diminished specific [3H]HCh-3 binding binding. These results suggest that the characteristics of the solubilized [3H]HCh-3 binding site are similar to those of the membrane-bound site.Keywords
This publication has 28 references indexed in Scilit:
- Effects of dipalmitoylphosphatidylcholine liposomes on high affinity transport of choline and synthesis of acetylcholine in cerebral cortical synaptosomes in the ratNeuropharmacology, 1987
- High-affinity, sodium-gradient-dependent transport of choline into vesiculated presynaptic plasma membrane fragments from the electric organ of Torpedo marmorata and reconstitution of the solubilized transporter into liposomesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1985
- A rapid filtration assay for soluble receptors using polyethylenimine-treated filtersAnalytical Biochemistry, 1983
- Inhibition of high affinity choline uptakeBiochemical Pharmacology, 1980
- High affinity choline transport and acetylCoA production in brain and their roles in the regulation of acetylcholine synthesisBrain Research Reviews, 1979
- THE UPTAKE, METABOLISM AND RELEASE OF HOMOCHOLINE: STUDIES WITH RAT BRAIN SYNAPTOSOMES AND CAT SUPERIOR CERVICAL GANGLIONJournal of Neurochemistry, 1977
- Regulation of Acetylcholine Synthesis: Does Cytoplasmic Acetylcholine Control High Affinity Choline Uptake?Science, 1976
- Synaptosomal transport and acetylation of 3-trimethylaminopropan-1-olBiochemical Pharmacology, 1976
- Neurochemical correlates of synaptically active amino acidsLife Sciences, 1974
- Role of Transmitter Uptake Mechanisms in Synaptic NeurotransmissionBritish Journal of Pharmacology, 1971