Nuclear matrix DNA from chicken erythrocytes contains beta-globin gene sequences.
- 1 January 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (2), 304-307
- https://doi.org/10.1073/pnas.81.2.304
Abstract
Nuclear matrices containing residual DNA were isolated from chicken erythrocytes after extraction of purine nuclei with buffered 2 M NaCl. After further purification of this residual DNA, it was found to contain high concentrations of .beta.-globin gene sequences as assayed by dot hybridization with 32P-labeled nick-translated pHB1001. EM of a random sample of this residual DNA fraction shows the DNA to be intimately associated with protein at various intervals. A hypothesis for enrichment of active genes in residual DNA from purified chromatin or in nuclear matrix DNA is also discussed.This publication has 36 references indexed in Scilit:
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