Resolution of circular, nicked circular and linear DNA, 4.4 kb in length, by electrophoresis in polyacrylamide solutions
- 1 January 1992
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 13 (1), 176-178
- https://doi.org/10.1002/elps.1150130136
Abstract
Circular DNA of more than 1,400 bp in size is known not to migrate into polyacrylamide gels. The migration of supercoiled plasmid pBR322 DNA (4,363 bp) into uncrosslinked polyacrylamide (MW 5 × 106) solutions and its separation, on the basis of conformation, from its nicked form is demonstrated in this study. Migration of the supercoiled, nicked circular and linear forms of the plasmid DNA is retarded in proportion to the concentration of uncrosslinked polyacrylamide, the degree of retardation being highest for the nicked circular form. Decreasing the level of supercoiling of the covalently closed circular form by decreasing the concentration of the intercalating dye (ethidium homodimer) shows that the degree of retardation decreases in proportion to the superhelix density.Keywords
This publication has 10 references indexed in Scilit:
- Information on DNA conformation derived from the Ferguson plot of DNA fragments of up to 9 kb in size, using polyacrylamide gel electrophoresis in a discontinuous buffer systemElectrophoresis, 1991
- Electrophoresis with intermittent scanning of the migration path: Detection of resolution within shortened timeElectrophoresis, 1991
- Electrophoretic size separations in liquified agarose of polystyrene particles and circular DNAElectrophoresis, 1991
- Separation of DNA restriction fragments by high performance capillary electrophoresis with low and zero crosslinked polyacrylamide using continuous and pulsed electric fieldsJournal of Chromatography A, 1990
- The electric field dependence of DNA mobilities in agarose gels: A reinvestigationElectrophoresis, 1990
- Properties of supercoiled DNA in gel electrophoresisJournal of Molecular Biology, 1986
- Electrophoresis and electrofocusing on polyacrylamide gel in the study of native macromoleculesMolecular and Cellular Biochemistry, 1980
- A Viscosity Model of Polyacrylamide Gel ElectrophoresisZeitschrift für Naturforschung C, 1979
- DNA bifunctional intercalators. 2. Fluorescence properties and DNA binding interaction of an ethidium homodimer and an acridine ethidium heterodimer. Appendix: Numerical solution of McGhee and von Hippel equations for competing ligandsBiochemistry, 1978
- Estimation of molecular radius, free mobility, and valence using polyacrylamide gel electrophoresisAnalytical Biochemistry, 1971