Quantification of vitamin C in the rat brain in vivo using short echo‐time 1H MRS
Open Access
- 3 April 2006
- journal article
- research article
- Published by Wiley in Magnetic Resonance in Medicine
- Vol. 55 (5), 979-983
- https://doi.org/10.1002/mrm.20854
Abstract
Both ultrashort echo‐time STEAM and MEGA‐PRESS‐edited spectroscopy were used to validate noninvasive quantification of vitamin C (ascorbate) in the developing rat brain, where changes in ascorbate concentration have been reported. Despite strong overlap with resonances from glutamine, glutamate, glutathione, and macromolecules, reliable quantification of ascorbate (Cramer‐Rao lower bounds < 0.2 μmol/g) by LCModel analysis of STEAM (TE = 2 ms) spectra was possible at 9.4 T. Ascorbate concentrations quantified from the STEAM spectra were in very good agreement with concentrations calculated from fully resolved ascorbate resonances in MEGA‐PRESS‐edited spectra measured from identical volumes of interest. Ascorbate concentrations measured using STEAM decreased with increasing postnatal rat age, in agreement with published brain ascorbate concentrations measured in vitro using high‐performance liquid chromatography (HPLC). Magn Reson Med, 2006.Keywords
This publication has 16 references indexed in Scilit:
- Highly resolved in vivo 1H NMR spectroscopy of the mouse brain at 9.4 TMagnetic Resonance in Medicine, 2004
- 1H NMR detection of vitamin C in human brain in vivoMagnetic Resonance in Medicine, 2004
- Developmental and regional changes in the neurochemical profile of the rat brain determined by in vivo 1H NMR spectroscopyMagnetic Resonance in Medicine, 2003
- Metabolic changes in quinolinic acid‐lesioned rat striatum detected non‐invasively by in vivo 1H NMR spectroscopyJournal of Neuroscience Research, 2001
- Field mapping without reference scan using asymmetric echo-planar techniquesMagnetic Resonance in Medicine, 2000
- Toward an in Vivo Neurochemical Profile: Quantification of 18 Metabolites in Short-Echo-Time 1H NMR Spectra of the Rat BrainJournal of Magnetic Resonance, 1999
- In vivo1H NMR spectroscopy of rat brain at 1 ms echo timeMagnetic Resonance in Medicine, 1999
- Differential compartmentalization of brain ascorbate and glutathione between neurons and gliaNeuroscience, 1997
- Estimation of metabolite concentrations from localized in vivo proton NMR spectraMagnetic Resonance in Medicine, 1993
- Automatic, localized in Vivo adjustment of all first‐and second‐order shim coilsMagnetic Resonance in Medicine, 1993