Clonal antibodies for core protein of chondroitin sulfate proteoglycan.
- 1 July 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (7), 3971-3973
- https://doi.org/10.1073/pnas.77.7.3971
Abstract
Antibodies were prepared to the core protein of cartilage chondroitin sulfate proteoglycan from clones isolated after fusion of rat spleen cells and mouse myeloma cell lines. Antibodies produced by 5 clones were studied in detail by a radioimmunoassay utilizing 35SO42--labeled hyaluronidase-digested chondroitin sulfate proteoglycan. Preparations of antigen contained at least 2 antigenic determinants, 1 restricted to a portion of antigen in these preparations. One clone reacted with proteoglycan synthesized in a cell-free system. Such clonal antibodies can be used for structural and biosynthetic studies of core protein.This publication has 12 references indexed in Scilit:
- Translation and characterization of messenger RNAs in differentiating chicken cartilage.Proceedings of the National Academy of Sciences, 1979
- Effect of reduction and alkylation on the antigenicity of cartilage proteoglycansBiochemical and Biophysical Research Communications, 1979
- Immunochemical analysis of cartilage proteoglycans. Antigenic determinants of substructuresBiochemical Journal, 1979
- Simultaneous localization of type II collagen and core protein of chondroitin sulfate proteoglycan in individual chondrocytes.Proceedings of the National Academy of Sciences, 1979
- Classes and subclasses of rat immunoglobulins: Interaction with the complement system and with staphylococcal protein AImmunochemistry, 1978
- A radioimmune study of the effect of bromodeoxyuridine on the synthesis of proteoglycan by differentiating limb bud culturesDevelopmental Biology, 1977
- Continuous cultures of fused cells secreting antibody of predefined specificityNature, 1975
- Aggregation of cartilage proteoglycans. II. Oligosaccharide competitors of the proteoglycan-hyaluronic acid interaction.1974
- The use of protein A‐containing Staphylococcus aureus as a solid phase anti‐IgG reagent in radioimmunoassays as exemplified in the quantitation of α‐fetoprotein in normal human adult serumEuropean Journal of Immunology, 1974
- Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, Ige and IgG1, IgG2a, IgG2b, IgG2cEuropean Journal of Immunology, 1974