Production and characterization of monoclonal antibodies against the Epstein-Barr virus membrane antigen
- 1 January 1982
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 41 (1), 258-264
- https://doi.org/10.1128/jvi.41.1.258-264.1982
Abstract
Five murine hybridoma lines that produce monoclonal antibodies against Epstein-Barr virus membrane antigen (MA) were established. Immunoprecipitation experiments demonstrated that three of the antibodies precipitated both the 236,000 (236K) MA and the 212K MA. The other two antibodies precipitated the 86K MA. Antibodies against the 236K-212K MA and the 86K MA mediated complement-dependent cytolysis of Epstein-Barr-virus-infected cells. The antibodies against the 86K MA neutralized both the B95-8 and P3HR-1 viruses. ImagesThis publication has 16 references indexed in Scilit:
- Radioimmune precipitation study comparing the epstein-barr virus membrane antigens expressed on P3HR-1 virus-superinfected Raji cells to those expressed on cells in a B-95 virus-transformed producer culture activated with tumor-promoting agent (TPA)Virology, 1980
- Analysis of early and late Epstein-Barr virus associated polypeptides by immunoprecipitationVirology, 1979
- Epstein‐Barr virus‐induced membrane antigens: Immunochemical characterization of triton X‐100 solubilized viral membrane antigens from EBV‐superinfected raji cellsInternational Journal of Cancer, 1979
- The isolation of hybrid cell lines producing monoclonal antibodies against the p15(E) protein of ecotropic murine leukemia virusesVirology, 1979
- Biochemical identification of primate lymphoid cell-surface glycoproteinsInternational Journal of Cancer, 1979
- Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3α,6α-diphenylglycolurilBiochemistry, 1978
- Persisting oncogenic herpesvirus induced by the tumour promoter TPANature, 1978
- Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphenylglycolurilBiochemical and Biophysical Research Communications, 1978
- Different surface glycoprotein patterns on human T‐, B‐ and leukemic‐lymphocytesInternational Journal of Cancer, 1976
- External Labeling of Cell Surface Galactose and Galactosamine in Glycolipid and Glycoprotein of Human ErythrocytesJournal of Biological Chemistry, 1973