Susceptibility of B‐cell deficient C57BL/6 (μMT) mice to Neospora caninum infection
- 1 May 1999
- journal article
- research article
- Published by Wiley in Parasite Immunology
- Vol. 21 (5), 225-236
- https://doi.org/10.1046/j.1365-3024.1999.00223.x
Abstract
Neospora caninum is a coccidian parasite of veterinary importance by causing abortion or stillbirth in cattle among other problems in diverse animal species. We assessed an experimental murine model for its suitability to study the immune response to N. caninum infection. Thus, wild-type (wt) C57BL/6 mice and B-cell (and consequently antibody)-deficient μMT mice were infected with N. caninum tachyzoites and sacrificed at days 10, 24 and 29–44 post infection (dpi). Various organs were collected for parasitological and pathological analysis, spleen and serum for immunological investigations. Splenocytes were in vitro-stimulated with N. caninum (NC)- and T. gondii-antigens for assessing T cell proliferation and cytokine production. While wt mice were resistant to disease, μMT mice died starting from 29 dpi onwards. Histological examination of brain tissue from μMT mice exhibited a high infection intensity with multifocal necrotic cerebral lesions, which were absent in the brains of wt mice. NC antigen-stimulated spleen cells of both wt and μMT mice infected with N. caninum showed a marked proliferative depression at 10 dpi. At 24 dpi, this immunosuppression was still maintained in μMT mice whereas it was restored in wt mice. Stimulated splenocytes of infected μMT mice secreted significantly less IFN-γ and less IL-10 than corresponding wt splenocytes. For IL-10, this difference increased with time. The susceptibility of μMT mice appeared associated to B-cell deficiency, allowing the persistant spread of the parasite causing immunosuppression and finally resulting in a lethal outcome of infection.Keywords
This publication has 17 references indexed in Scilit:
- Differential Effects of LPS and CD40 Ligand Stimulations on the Induction of IL-12 Production by Dendritic Cells and MacrophagesCellular Immunology, 1997
- Th1-Th2: Reliable paradigm or dangerous dogma?Immunology Today, 1997
- Cytokines acting on or secreted by macrophages during intracellular infection (IL-10, IL-12, IFN-γ)Current Opinion in Immunology, 1997
- Function and clinical use of interleukin-12Current Opinion in Hematology, 1997
- Adhesion and invasion of bovine endothelial cells byNeospora caninumParasitology, 1996
- NeosporosisParasitology Today, 1993
- Towards a comprehensive view of immunoglobulin class switchingImmunology Today, 1993
- A B cell-deficient mouse by targeted disruption of the membrane exon of the immunoglobulin μ chain geneNature, 1991
- The neuropathology in toxoplasmosis‐like infection caused by a newly recognized cyst‐forming sporozoon in dogsAPMIS, 1989
- Dyes as Microchemical Indicators of a New Immunity Phenomenon Affecting a Protozoon Parasite (Toxoplasma)Science, 1948