In vitrostudy of TMAO reduction byShewanella putrefaciensisolated from cod fillets packed in modified atmosphere
- 1 February 1998
- journal article
- research article
- Published by Taylor & Francis in Food Additives & Contaminants
- Vol. 15 (2), 229-236
- https://doi.org/10.1080/02652039809374634
Abstract
A strain of Shewanella putrefaciens was isolated from cod fillets in a modified atmosphere (60% CO2, 30% O2, 10% N2). The effect of pH on the growth of Shewanella putrefaciens and TMAO reduction was studied in fish extract with citrate buffer systems at pH values from 5.8 to 6.8. The effect of the modified atmosphere (CO2, O2) on the growth of S. putrefaciens and on the reduction of TMAO to TMA was studied using solid media from fish extract packed under variable mixtures of CO2, O2 and N2 . All the samples were incubated at 7°C for 7 days. Slow development of S. putrefaciens and no production of TMA was observed in fish extract buffered at pH 5.8. Inclusion of O2in the packaging atmosphere of solid fish extract, even at small proportions (10%), was inhibitory for the reduction of TMAO, while there was no effect of O2on the growth of S. putrefaciens. Increased levels of CO2 (≥ 50%) in the atmosphere were inhibiting the growth of S. putrefaciens and consequently the production of TMA.Keywords
This publication has 10 references indexed in Scilit:
- Qualitative and quantitative characterization of spoilage bacteria from packed fishInternational Journal of Food Microbiology, 1995
- New Method for Analysis of Trimethylamine Oxide Using Ferrous Sulfate and EDTAJournal of Food Science, 1991
- Modified atmosphere packaging of seafoodCritical Reviews in Food Science and Nutrition, 1990
- Growth and activity of Shewanella putrefaciens isolated from spoiling fishInternational Journal of Food Microbiology, 1989
- Detection of specific spoilage bacteria from fish stored at low (0°C) and high (20°C) temperaturesInternational Journal of Food Microbiology, 1987
- Phylogeny of the Vibrionaceae, and Recommendation for Two New Genera, Listonella and ShewanellaSystematic and Applied Microbiology, 1985
- Microbial Flora of Cod Fillets (Gadus morhua) Stored at 2°C in Different Mixtures of Carbon Dioxide and Nitrogen/OxygenJournal of Food Protection, 1985
- A conductance method for the assay and study of bacterial trimethylamine oxide reductionJournal of Applied Bacteriology, 1982
- A Numerical Taxonomic Study of Some Pseudomonas-like Marine BacteriaJournal of General Microbiology, 1977
- A rapid selective medium for the determination of trimethylamineoxide-reducing bacteriaJournal of Basic Microbiology, 1974