Interference screw fixation of four-strand hamstring grafts for ACL reconstruction has recently been introduced. By this method, the interference screw is placed in the tibial and femoral tunnels eccentric (adjacent) to the bundled limbs of the graft. In order to maximize the graft to tunnel contact to promote ¶biological fixation, it is proposed to place the screw concentrically in ¶the tunnel, in the middle of the four limbs of the graft, pressing each limb of the graft into the tunnel wall. This would be difficult to do in the proximal, folded end of the four limb graft situated in the femoral tunnel but can be done easily in the tibial tunnel. The purpose of this study was to evaluate the effect of screw placement on the stiffness, yield load, and ultimate load of hamstring graft fixation in the tibial tunnel. Five pairs of human knees were ¶used for the study. Pull out tests ¶were performed using an MTS system, pulling along the axis of the ¶tibial tunnel. Tibial fixation stiffness was greater using concentric screw placement (P < 0.05) although there was no statistical difference in yield load, slippage, or ultimate load.