Localization of Radioiodinated Antibodies in Rats Bearing Tumors Induced by N-2-Fluorenylacetamide23

Abstract

Liver tumors, induced in rats by N-2-fluorenylacetamide (N-2-FAA), localized antibodies which had been prepared in rabbits against like tumors. The localization was followed by radioiodinating the antibodies and injecting them intravenously. These tumors also localized antibodies against normal liver. They did not, as a rule, localize antifibrin antibodies or fibrinogen during the 18 hours between injection and assay. In general they competed well with normal organs, such as liver, for circulating antibodies. In all these respects and by the ease with which they could be perfused, liver tumors induced by N-2-FAA sharply contrasted with transplanted tumors. In addition, radiolabeled antibodies which had localized in such induced tumors could be recovered from them by elution and returned to like tumors by the intravenous route.