Molecular cloning and characterization of a gene coding for methanol oxidase inHansenula polymorpha

Abstract

The structural gene and the regulatory DNA sequence of the yeast Hansenula polymorpha methanol oxidase have been isolated. According to the nucleotide sequence data obtained, the structural gene encodes a 664 amino acids long protein, contains no intervening sequences, and the 5′- and 3′-non-coding region contains several sequences implicated in transcription initiation and termination in the yeast Saccharonyces cerevisiae Although the methanol oxidase is tranelocated to the peroxisomes, no cleavable signal sequence was found at the N-terminus of the protein.