CHEMICALLY DEFINED MEDIUM FOR GROWTH OF MICROCOCCUS LYSODEIKTICUS

Abstract

A chemically defined medium for growth of M. lysodeikticus is presented. The organism possesses a relatively nonspecific but absolute purine requirement that can best be satisfied by the free base hypoxanthine although adenine also allows some growth. A substitution for hypoxanthine, however, can be made by inosine, adenosine, or adenylic acid, but not by guanosine or guanylic acid. Although biotin stimulates growth, equally good growth occurs using biocytin or biotiu-d-sulphoxide. Less stimulation is apparent using desthiobiotin, dl-oxybiotin, or biotirt-l-sulphoxide. Although amino acids are necessary for growth, no absolute requirement for a specific amino acid can be demonstrated. The amino acid requirements need to be defined in terms of those amino acids which support good growth in the presence or absence of glutamic acid.