Chemical synthesis of insulin‐like growth factor II
- 1 September 1985
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 26 (3), 299-304
- https://doi.org/10.1111/j.1399-3011.1985.tb03208.x
Abstract
Human insulin-like growth factor II (IGF-II) with 67 amino acids and three disulfide bridges has been synthesized by the solid-phase method. The synthetic hormone is shown to be homogeneous in high performance liquid chromatography (HPLC), high performance partition chromatography (HPPC), and chromatofocusing. It is indistinguishable from natural hormone in HPLC, peptide map of thermolysin digests, amino acid composition and radioreceptor binding assay. Thus, synthetic and natural IGF-II are identical.Keywords
This publication has 28 references indexed in Scilit:
- Synthesis and properties of human β-endorphin-(1-9) and its analogsInternational Journal of Peptide and Protein Research, 2009
- High-performance partition chromatography of peptides on a new cross-linked agaroseInternational Journal of Peptide and Protein Research, 1983
- PEPTIDE SEGMENT COUPLING IN AQUEOUS MEDIUM: SILVER ION ACTIVATION OF THE THIOLCARBOXYL GROUPInternational Journal of Peptide and Protein Research, 1981
- A new synthetic route to tert-butyloxycarbonylaminoacyl-4-(oxymethyl)phenylacetamidomethyl-resin, an improved support for solid-phase peptide synthesisThe Journal of Organic Chemistry, 1978
- Primary structure of human insulin‐like growth factor IIFEBS Letters, 1978
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- CONFORMATIONAL STUDIES ON PROTEINS BY ISOELECTRIC FOCUSING*Annals of the New York Academy of Sciences, 1973
- Synthèse du glutathion et de l'oxytocine à l'aide d'un nouveau groupe protecteur de la fonction thiolHelvetica Chimica Acta, 1966
- Solid Phase Peptide Synthesis. I. The Synthesis of a TetrapeptideJournal of the American Chemical Society, 1963
- Preparation of Iodine-131 Labelled Human Growth Hormone of High Specific ActivityNature, 1962