Abstract
Usual spp. of rumen protozoa and certain bacteria, being used as indicators of the presence of characteristic rumen flora, readily were established in the rumens of 6 calves which were given rumen inoculations with cud materials and access to lawn pasture during their first 6 wks. of age. None of the protozoa and only limited numbers of one var. of the microflora became established in 6 similarly fed but uninoculated calves during the same period. The same microorganisms became established only in relatively limited numbers in a 2-mo.-old uninoculated calf on pasture for 7 wks. with 4 rumeninoculated calves of similar age. A rumen inoculation rectified the difference in microorganisms. Prior to inoculation, this calf had recurrent mild diarrhea and a comparatively rough hair coat but percentage gain in wt. was only slightly less than an inoculated twin.