Authentic beta-globin mRNA sequences in homozygous betaO-thalassemia.
- 1 July 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (7), 3047-3051
- https://doi.org/10.1073/pnas.74.7.3047
Abstract
In a patient with homozygous .beta.0-thalassemia in whom studies of reticulocyte Hb synthesis showed no .beta.-globin chain synthesis in vivo and in vitro, molecular hybridization studies revealed RNA sequences complementary to .beta.-globin c[complementary]DNA. The fact that these sequences were authentic .beta.-globin mRNA was shown by fingerprint analysis of T1 ribonuclease-digested mRNA and by sequencing of oligonucleotides unique to .beta.-globin mRNA. The .beta.-mRNA that failed to direct .beta.-globin chain synthesis was not detectably shortened or degraded and contained poly(A) sequences.This publication has 30 references indexed in Scilit:
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