Chemical probes for tRNA tertiary structure
- 27 December 1982
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 150 (2), 459-464
- https://doi.org/10.1016/0014-5793(82)80789-8
Abstract
The tertiary structure of tRNA in solution can be proved by chemical modification experiments. Three reagents, N-ethyl-N-nitrosourea, N-methyl-N-nitrosourea and dimethylsulfate which are known to alkylate nucleic acids at nucleophilic centers were compared. It is found that N-ethyl-N-nitrosourea and N-methyl-N-nitrosourea mainly react with phosphate residues and dimethylsulfate only with the bases. With dimethylsulfate the extent of alkylation of guanosines is about one order of magnitude higher than that of the phosphates by the nitrosocompounds.Keywords
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