Proportionate representation of rDNA and Balbiani ring DNA in polytene chromosomes of Chironomus tentans
- 1 January 1976
- journal article
- research article
- Published by Springer Nature in Chromosoma
- Vol. 57 (2), 185-197
- https://doi.org/10.1007/bf00292917
Abstract
Attention was focussed on the question whether the most active genes of the polytene genome of Chironomus tentans, i.e. rDNA and Balbiani rings, have undergone the same number of duplications as the bulk of the genome. RNA was extracted from isolated salivary glands, enriched for messenger RNA by poly U sepharose chromatography, and labelled with 125Iodine. In situ hybridization showed that, apart from rRNA, Balbiani ring 1 and 2 transcripts are the major hybridizing RNA species present in this enriched preparation. BR1 hybridized only at the b-region. Transcripts of BR6 and puff I 17B are shown to be salivary gland specific and hybridize in situ to an extent of less than 5% of the RNA hybridized to BR1 plus BR2. Saturation hybridization levels of rRNA and Balbiani ring (BR1 plus BR2) RNA with polytene DNA from salivary glands were determined and compared with the saturation levels measured for DNA from adult flies and larvae. rRNA hybridized to the same level (0.09%) with polytene DNA as with DNA from primarily diploid cells (adult fly DNA) or from larvae. An equal hybridization level (0.04%) with each of these three types of DNA was also obtained for BR RNA. The results demonstrate that, during polytenization in C. tentans, the genes for rRNA and BR1 and 2 are duplicated to the same extent as the bulk of the genome.Keywords
This publication has 34 references indexed in Scilit:
- Transcription in polytene chromosomesCell, 1975
- Polyploidization of the silk gland of Bombyx moriJournal of Molecular Biology, 1974
- Quantitative micro-assay for RNA/DNA hybrids in the study of nucleolar RNA from Chironomus tentans salivary gland cellsExperimental Cell Research, 1973
- The genes for silk fibroin in Bombyx moriJournal of Molecular Biology, 1972
- IN SITU DEMONSTRATION OF DNA HYBRIDIZING WITH CHROMOSOMAL AND NUCLEAR SAP RNA IN CHIRONOMUS TENTANS The Journal of cell biology, 1972
- Isolation and Identification of the messenger RNA for silk fibroin from Bombyx moriJournal of Molecular Biology, 1972
- Differential representation of chromosomal HRNA in nuclear sapExperimental Cell Research, 1971
- The Content of Deoxyribonucleic Acid in Individual Polytene Chromosomes of Chironomus tentansCytogenetic and Genome Research, 1967
- A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membraneJournal of Molecular Biology, 1965
- Chromomerenkonstanz und spezifische Modifikationen der Chromosomenstruktur in der Entwicklung und Organdifferenzierung von Chironomus tentansChromosoma, 1953