Combined FISH Painting and Harlequin Staining for Cell Cycle-controlled Chromosome Analysis in Human Lymphocytes
- 1 January 1995
- journal article
- research article
- Published by Taylor & Francis in International Journal of Radiation Biology
- Vol. 68 (1), 25-27
- https://doi.org/10.1080/09553009514550881
Abstract
We present a simple method that allows scoring of FISH-painted chromosomes exclusively in the first division human lymphocytes. It consists of a combination of FISH with chromosome-specific libraries and a differential sister chromatid staining after BrdU treatment of lymphocyte cultures. The method allows a precise quantification of induced chromosome damage for human biodosimetry.Keywords
This publication has 12 references indexed in Scilit:
- Fish “painting” patterns resulting from complex exchangesMutation Research/Environmental Mutagenesis and Related Subjects, 1994
- Construction of Mouse Chromosome-specific DNA Libraries and Their Use for the Detection of X-ray-induced AberrationsInternational Journal of Radiation Biology, 1994
- Radiation-induced Chromosome Aberrations Analysed by Two-colour Fluorescencein SituHybridization with Composite Whole Chromosome-specific DNA Probes and a Pancentromeric DNA ProbeInternational Journal of Radiation Biology, 1993
- Rapid Translocation Frequency Analysis in Humans Decades after Exposure to Ionizing RadiationInternational Journal of Radiation Biology, 1992
- The persistence of chromosome translocations in a radiation worker accidentally exposed to tritiumCytogenetic and Genome Research, 1992
- Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4.Proceedings of the National Academy of Sciences, 1988
- Dependence of the frequency of harlequin-stained cells on BrdU concentration in human lymphocyte culturesMutation Research Letters, 1981
- Azathioprine, a clastogen in human somatic cells? Analysis of chromosome damage and SCE in lymphocytes after exposure in vivo and in vitroMutation Research/Genetic Toxicology, 1981
- Analysis of human lymphocyte cell cycle time in culture measured by sister chromatid differential stainingExperimental Cell Research, 1977
- New Giemsa method for the differential staining of sister chromatidsNature, 1974