Replication of DNA by Nuclei Isolated from Soybean Suspension Cultures
- 1 October 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 66 (4), 726-730
- https://doi.org/10.1104/pp.66.4.726
Abstract
DNA replication was studied in nuclei isolated from soybean cells grown in suspension culture. The isolation procedure involved the preparation of protoplasts, their lysis with a nonionic detergent and purification of nuclei. These nuclei synthesized low MW DNA and joined these fragments into DNA of intermediate MW. The characteristics of replication in isolated nuclei correlated well with those of the cells from which they were isolated, as shown by fluorodeoxyuridine synchronization and UV irradiation experiments.This publication has 9 references indexed in Scilit:
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