Gazaniaxanthin, a new xanthophyll of the formula C40H56O or C40H54O, was isolated from the petals of Gazania rigens. Its spectral behavior is similar to that of [gamma]-carotene and rubixanthin, the absorption maxima being slightly displaced to shorter wave lengths. Besides gazaniaxanthin there occur in the flowers of G. rigens rubixanthin, lutein and "leaf xanthophyll," which were isolated in pure crytalline state, and probably [gamma]-carotene. Another unknown carotenoid with the same absorption spectrum as [gamma]-carotene could not be isolated in crystalline state.