Microbiological Assay Method for Thiamine Using Lactobacillus fermenti 36 as Test Organism.

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Abstract
Maltose, takadias-tase-digested starch and ascorbic acid stimulate the early growth of Lactobacillus fermenti 36 in microbiological assay medium containing thiamine in suboptimal or optimal amt. The stimulatory effect or maltose or enzyme-digested starch increased with increasing amts. up to 20 mg./10 ml. thiamine assay medium. Maltose or enzyme-digested starch is also stimulatory to L. arabinosus 17-5 and Streptococcus faecalis R in assays of panto-thenic acid and pyridoxine. Precaution should be taken in the thiamine assay of starchy substances with L. fermenti 36. Addition of 20 mg. of enzyme-digested starch or maltose to 10 ml. of assay medium is recommended.