A readily synthesized cyclic pyrrolysine analogue for site-specific protein “click” labeling
- 9 March 2011
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Chemical Communications
- Vol. 47 (15), 4502-4504
- https://doi.org/10.1039/c1cc00024a
Abstract
A concise route was developed for the facile synthesis of a cyclic pyrrolysine analogue bearing an azide handle. Directed evolution enabled the encoding of this non-natural amino acid in both prokaryotic and eukaryotic cells, which offers a highly efficient approach for the site-specific protein labeling using click chemistry.Keywords
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