Studies on the State of Insulin in Blood: Properties of Circulating “Free” and “Bound” Insulin
- 1 April 1965
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 76 (4), 709-721
- https://doi.org/10.1210/endo-76-4-709
Abstract
Studies are presented on the immunologic reactivity, electrophoretic mobility on Pevikon and chromatographic behavior on Sephadex of the 2 forms of insulin in human blood. The “free” form of insulin corresponds in its physicochemical, immunologic and biological properties to crystalline insulin. The “bound” form of insulin exhibits a higher molecular weight, slower electrophoretic mobility and diminished reactivity with insulin antisera. Acid ethanol extraction increases the reactivity of “bound” insulin with insulin antisera, the antisera inhibiting the biological activity of acid ethanol extracts of “bound” insulin on isolated epididymal adipose tissue. (Endocrinology76: 709, 1965)Keywords
This publication has 23 references indexed in Scilit:
- MEASUREMENT OF SMALL QUANTITIES OF INSULIN-LIKE ACTIVITY USING RAT ADIPOSE TISSUE. II. EVALUATION OF PERFORMANCE*JCI Insight, 1960
- MEASUREMENT OF SMALL QUANTITIES OF INSULIN-LIKE ACTIVITY USING RAT ADIPOSE TISSUE. I. A PROPOSED PROCEDURE*JCI Insight, 1960
- STUDIES ON THE METABOLISM OF ADIPOSE TISSUE .1. EFFECT OF INSULIN ON GLUCOSE UTILIZATION AS MEASURED BY THE MANOMETRIC DETERMINATION OF CARBON DIOXIDE OUTPUT1959
- ELECTROPHORETIC SEPARATION OF INSULIN ACTIVITY OF NORMAL PLASMA AND PLASMA WITH ADDED INSULIN-I-1311959
- INSULIN-LIKE ACTIVITY OF HUMAN PLASMA CONSTITUENTS .3. ELUTION OF INSULIN-LIKE ACTIVITY FROM CATIONIC EXCHANGE RESINS1958
- INSULIN-LIKE ACTIVITY OF HUMAN PLASMA CONSTITUENTS .2. BIOLOGIC ASSAY OF HUMAN PLASMA FRACTIONS FOR INSULIN-LIKE ACTIVITY1956
- INSULIN-LIKE ACTIVITY OF HUMAN PLASMA CONSTITUENTS .1. DESCRIPTION AND EVALUATION OF BIOLOGIC ASSAY FOR INSULIN-LIKE ACTIVITY1956
- SEDIMENTATION BEHAVIOR OF PROTEINS AND OTHER MATERIALS IN A HORIZONTAL PREPARATIVE ROTORJournal of Biological Chemistry, 1954
- ESTIMATION OF PLASMA-INSULIN BY THE RAT DIAPHRAGM METHODThe Lancet, 1954
- THE INSULIN AND THE ZINC CONTENT OF NORMAL AND DIABETIC PANCREASJCI Insight, 1938