Characterization of cytoplasmic and nuclear genomes in the colorless alga Polytoma. I. Ultrastructural analysis of organelles.
Open Access
- 1 May 1976
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 69 (2), 352-370
- https://doi.org/10.1083/jcb.69.2.352
Abstract
EM studies have been made on the fine structure of the colorless biflagellate, P. obtusum, with main emphasis on the structural organization of the mitochondria and the leucoplast. Both organelles have been demonstrated to contain DNA aggregates as well as ribosomal particles within their matrix material. Reconstructions from serial sections showed that the mitochondria were highly convoluted and irregular in shape and size, and the leucoplast was a single cup-shaped entity, with large starch grains, localized at the posterior end and multiple sites of DNA aggregates. The starch-containing compartments appeared to be interconnected by narrow tubular or sheetlike bridges. Cytoplasmic invaginations into the plastid, often containing mitochondria, were of frequent occurrence and membranes of mitochondria and the leucoplast appeared to be closely apposed. Membranous elements, both sheetlike and vesicular, were also present in the matrix. The Polytoma leucoplast was, in certain respects, morphologically similar to the plastids of various photosynthetic mutants of Chlamydomonas, most of which show Mendelian segregation. It is suggested that Polytoma arose from Chlamydomonas-like ancestor, possibly through combined mutational processes of both chloroplast and nuclear genomes. Since Polytoma leucoplasts contain both DNA and ribosomal particles, it is probable that these organelles still possess semiautonomy and limited ability for protein synthesis.This publication has 29 references indexed in Scilit:
- Denaturation and the renaturation kinetics of chloroplast DNA from Chlamydomonas reinhardiJournal of Molecular Biology, 1971
- THE GENETICS OF PHOTOSYNTHESIS AND OF THE CHLOROPLAST IN CHLAMYDOMONAS REINHARDIAnnual Review of Genetics, 1970
- CHLOROPLAST STRUCTURE AND FUNCTION IN ac-20, A MUTANT STRAIN OF CHLAMYDOMONAS REINHARDI The Journal of cell biology, 1970
- Characterization of the chloroplastic and cytoplasmic ribosomes of Chlamydomonas reinhardiJournal of Molecular Biology, 1969
- NUCLEIC ACIDS OF CHLOROPLASTS AND MITOCHONDRIA IN SWISS CHARDThe Journal of cell biology, 1965
- Satellite DNA components in Euglena gracilis cells lacking chloroplastsJournal of Molecular Biology, 1965
- The general occurrence of mitochondrial DNAExperimental Cell Research, 1965
- Electron‐Microscopic Demonstration of Plastids in Polytoma*The Journal of Protozoology, 1963
- ELECTRON MICROSCOPE STUDIES ON BLUE-GREEN ALGAEThe Journal of cell biology, 1961
- STRUCTURE AND DEVELOPMENT OF THE CHLOROPLAST IN CHLAMYDOMONASThe Journal of cell biology, 1957