Systemic inflammation increases shear stress-induced platelet plug formation measured by the PFA-100
- 1 December 2000
- journal article
- clinical trial
- Published by Wiley in British Journal of Haematology
- Vol. 111 (4), 1250-1252
- https://doi.org/10.1046/j.1365-2141.2000.02473.x
Abstract
The PFA-100 measures platelet plug formation under shear stress and is strongly dependent on von Willebrand Factor (VWF) levels in plasma. We therefore hypothesized that elevated VWF levels, possibly as a result of acute inflammation, adversely influence PFA-100 results. Healthy volunteers received either 2 ng/kg endotoxin or placebo in a randomized controlled trial. Four hours after endotoxin (but not placebo) infusion VWF levels increased by 85%, collagen epinephrine-induced closure time (CT) decreased by 47% and collagen ADP-CT decreased by 38% (P < 0.0001) respectively. In conclusion, systemic inflammation has a major impact on the results obtained by PFA-100 and may confound interpretation of platelet function.Keywords
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