Homogeneity of antibody-producing cells as analysed by their buoyant density in gradients of Ficoll.
- 1 November 1970
- journal article
- Vol. 19 (5), 817-29
Abstract
Ficoll, a polymer of sucrose, has several advantages over proteins for preparation of density gradients used to study mammalian cells. In addition to its ease of preparation, Ficoll as a result of being an uncharged molecule, does not bind ions from solution and, therefore, does not affect the osmolality of the medium in which it is dissolved.This publication has 13 references indexed in Scilit:
- Separation of cells by velocity sedimentationJournal of Cellular Physiology, 1969
- Density Distribution Analysis of Cells Forming 19 S Hemolytic Antibody in the RatThe Journal of Immunology, 1969
- THE SEPARATION OF DIFFERENT CELL CLASSES FROM LYMPHOID ORGANSImmunology & Cell Biology, 1968
- THE SELECTIVE ELIMINATION OF IMMUNOLOGICALLY COMPETENT CELLS FROM BONE MARROW AND LYMPHATIC CELL MIXTURES II. MOUSE SPLEEN CELL FRACTIONATION ON A DISCONTINUOUS ALBUMIN GRADIENTTransplantation, 1968
- THE MORPHOLOGY OF ANTIBODY‐FORMING CELLS IN THE MOUSEImmunology & Cell Biology, 1968
- The Effect of pH on the Volume, Density and Shape of Erythrocytes and Thymic Lymphocytes*British Journal of Haematology, 1968
- Concentration, quantitation, and identification of hemopoietic stem cells.Proceedings of the National Academy of Sciences, 1967
- ELECTRON MICROSCOPIC OBSERVATIONS ON ANTIBODY-PRODUCING LYMPH NODE CELLSThe Journal of Experimental Medicine, 1966
- Membrane properties of living mammalian cells as studied by enzymatic hydrolysis of fluorogenic esters.Proceedings of the National Academy of Sciences, 1966
- Use of an Antiglobulin Serum to detect Cells producing Antibody with Low Haemolytic EfficiencyNature, 1965