A Modified Semi-automated Resorcinol Method for the Determination of Inulin

Abstract

A semi-automated resorcinol method for the determination of inulin in plasma and urine specimens, using standard AutoAnalyzer modules, is described. Plasma and other protein-containing fluids undergo a preliminary manual protein precipitation procedure. The deproteinized specimens are processed at a rate of 50/hr, with a high degree of accuracy and precision. The superiority of the present method is largely due to the elimination of small acid-resistant pump tubes from the manifold. This is possible because reagents are premixed prior to aspiration into the system, allowing simplification of manifold design over other automated methods.