Determination of Avidin by Use of Radioactive Biotin and Sephadex Chromatography.
- 1 October 1964
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 117 (1), 17-20
- https://doi.org/10.3181/00379727-117-29485
Abstract
Summary A method for determination of avidin by use of radioactive biotin and a gel-filtration technique has been developed. The method is based on measurement of the radioactivity of the avidin-biotin complex which separates from free biotin when a reaction solution of avidin and excess radioactive biotin is applied to a Sephadex G-25 column.Keywords
This publication has 1 reference indexed in Scilit:
- AVIDIN. 2. PURIFICATION AND COMPOSITIONBiochemical Journal, 1963