Ca2+/calmodulin‐dependent protein kinase in Saccharomyces cerevisiae
- 13 July 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 219 (1), 249-253
- https://doi.org/10.1016/0014-5793(87)81226-7
Abstract
Ca2+‐dependent chromatography of soluble cytosolic proteins on calmodulin‐Sepharose gave a fraction that exhibited Ca2+‐ and calmodulin‐dependent phosphorylation of several polypeptides, including 60, 56 and 45 kDa species. At 0.2 μM beef calmodulin the phosphorylation was optimal at 3 μM free Ca2+, and at 80 μM free Ca2+ it was half‐maximal at about 0.1 μM beef calmodulin. It is concluded that the fraction contains calmodulin‐dependent protein kinase(s) which is (are) autophosphorylated or associated with substrates.Keywords
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