Radioimmunoassay of androstenedione and testosterone in cow plasma at the time of luteolysis and oestrus

Abstract
Androstenedione and testosterone were measured by radioimmunoassay after chromatography on micro-columns of Lipidex-5000 in jugular plasma samples taken every 2-3 h at the time of luteolysis and estrus in 3 dairy cows. The concentrations of androstenedione and testosterone varied between 5 and 60 pg/ml and 5 and 80 pg/ml, respectively, and no consistent pattern in the fluctuations of either steroid was observed.