KAR1-MEDIATED TRANSFORMATION OF BREWING YEAST
Open Access
- 4 March 1991
- journal article
- research article
- Published by The Institute of Brewing & Distilling in Journal of the Institute of Brewing
- Vol. 97 (2), 115-118
- https://doi.org/10.1002/j.2050-0416.1991.tb01060.x
Abstract
Transfer of plasmid between nuclei has been observed in heterokaryons obtained from kar1 × KAR1 crosses3. On this basis, brewing yeast strain CC45 was forced to mate with K5-5A, a kar1 laboratory strain bearing plasmid YEpCR21. This multicopy plasmid carries the gene cyh2 and determines a dominant resistance to cycloheximide. Heteroplasmons having a CC45 nucleus and hosting YEpCR21 were obtained. Plasmid stability and brewing performance have been studied. The utility of kar1-mediated plasmid transference as an alternative method to transform brewing yeast is discussed. Advantages of YEpCR21 as a vector cloning for brewing yeast are considered as well.This publication has 5 references indexed in Scilit:
- Characterization of the transmission during cytoductant formation of the 2 μm DNA plasmid from SaccharomycesMolecular Genetics and Genomics, 1981
- Internuclear transfer of genetic information in kar1-1/KAR1 heterokaryons in Saccharomyces cerevisiae.Molecular and Cellular Biology, 1981
- Altered ribosomal protein L29 in a cycloheximide-resistant strain of Saccharomyces cerevisiaeCurrent Genetics, 1980
- A mutant of Saccharomyces cerevisiae defective for nuclear fusion.Proceedings of the National Academy of Sciences, 1976
- Gas Chromatographic Determination of Beer Volatiles by Carbon Disulfide Extraction: Improved Methodology, Data Handling, and InterpretationJournal of the American Society of Brewing Chemists, 1976