Three Approaches to the Radioimmunoassay of Human β-Thromboglobulin

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Abstract
The standard method, using antiserum in solution, could be used to measure plasma concentrations of .beta.-thromboglobulin with the results available after 2-3 days. The use of a greater dilution of antiserum and tracer, with delayed addition of tracer, resulted in a more sensitive assay suitable for measuring .beta.-thrombogbobulin in urine. The use of a solid-coupled antiserum under non-equilibrium conditions allowed the measurement of plasma levels of .beta.-thromboglobulin after an assay incubation time of 1 h. These 3 radioimmunoassay systems for .beta.-thromboglobulin cover the likely clinical requirements for the measurement of this platelet specific protein.