Cytoplasmic inheritance in Saccharomyces cerevisiae: Comparison of zygotic mitochondrial inheritance patterns
- 1 January 1976
- journal article
- conference paper
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 144 (3), 289-299
- https://doi.org/10.1007/bf00341727
Abstract
Mitochondrial movements in Saccharomyces cerevisiae (Sc) zygotes were monitored with phase-contrast microscopy and compared to known mitochondrial inheritance systems. The mitochondria of Sc were convincingly identified by integrated use of phase-contrast, cytochemical and electron microscopic observations. Mitochondria in Sc appear to move by saltatory jumps, which appear to be oriented towards movement of mitochondria into developing buds. Tracking of mitochondria of different genotypes was made possible by positive identification of each mitochondrial population before zygosis, and by the low degree of mixing (<10%) of mitochondrial populations before first bud septation. A grande by grande cross demonstrated equal numbers of mitochondria from each haploid moving into the first zygotic bud. A grande by neutral petite cross gave a 2:1 ratio of grande to petite mitochondria. However, a grande by suppressive petite cross gave equal numbers of grande and petite mitochondria. Using drug resistance systems, a comparison was made of highly biased (97%) and moderately biased (71%) chloramphenicol resistant inheritance patterns. In both cases, the ratios of drug resistant to sensitive mitochondria were 1:1. When numbers of mitochondria moving into an individual bud were compared to the phenotypic content of the clone of that bud, no model could be constructed which could predict the latter from the former. The data indicate (with the exception of the neutral petite by grande cross) that the numbers of each mitochondrial type “inserted” into the first zygotic bud are equal, regardless of the degree of asymmetry of inheritance of mitochondrial markers.This publication has 36 references indexed in Scilit:
- Mitochondrial MorphologyScience, 1974
- NUCLEAR GENE DOSAGE EFFECTS ON MITOCHONDRIAL MASS AND DNAThe Journal of cell biology, 1974
- Mitochondrion of Yeast: Ultrastructural Evidence for One Giant, Branched Organelle per CellScience, 1973
- THE ASSOCIATION OF A CLASS OF SALTATORY MOVEMENTS WITH MICROTUBULES IN CULTURED CELLSThe Journal of cell biology, 1970
- A low-viscosity epoxy resin embedding medium for electron microscopyJournal of Ultrastructure Research, 1969
- A FIBER APPARATUS IN THE NUCLEUS OF THE YEAST CELLThe Journal of cell biology, 1966
- HISTOCHEMICAL STUDIES OF MITOCHONDRIAL VARIATION DURING AEROBIC GROWTH OF RESPIRATION-NORMAL BAKER'S YEASTJournal of Histochemistry & Cytochemistry, 1965
- A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPYThe Journal of cell biology, 1965
- THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPYThe Journal of cell biology, 1963
- HISTOCHEMICAL DEMONSTRATION OF CYTOCHROME OXIDASE WITH NEW AMINE REAGENTSJournal of Histochemistry & Cytochemistry, 1960