STUDIES ON THE BIOASSAY OF HORMONES THE ASSAY OF TESTOSTERONE PROPIONATE AND ANDROSTERONE BY A CHICK COMB INUNCTION METHOD1

Abstract

Previously Frank et al. (1942) described a chick comb method for the assay of androgens. This communication is concerned with the application of this method to the assay of androsterone and testosterone propionate. animals, methods, materials White Leghorn male and female chicks obtained from the Kerr Chickeries, Frenchtown, New Jersey were employed in this study. They were shipped from the hatchery on the day of hatching and received in Cleveland the following day at the age of 1 to 2 days. The following day, at the age of to 3 days, inunctions were started and continued once daily for 7 days. The total dose of androgens was applied to the comb in a total of 0.35 cc. corn oil. The assays were carried out as described in detail by Frank et al. 1942). The androsterone and testosterone propionate were kindly supplied by Ciba Pharmaceutical Products, Inc.