Mechanism for Regulating Cell Surface Distribution of Na + ,K + -ATPase in Polarized Epithelial Cells
- 8 November 1991
- journal article
- editorial
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 254 (5033), 847-850
- https://doi.org/10.1126/science.1658934
Abstract
Restriction of sodium, potassium adenosine triphosphatase (Na+,K(+)-ATPase) to either the apical or basal-lateral membrane domain of polarized epithelial cells is fundamental to vectorial ion and solute transport in many tissues and organs. A restricted membrane distribution of Na+,K(+)-ATPase in Madin-Darby canine kidney (MDCK) epithelial cells was found experimentally to be generated by preferential retention of active enzyme in the basal-lateral membrane domain and selective inactivation and loss from the apical membrane domain, rather than by vectorial targeting of newly synthesized protein from the Golgi complex to the basal-lateral membrane domain. These results show how different distributions of the same subunits of Na+,K(+)-ATPase may be generated in normal polarized epithelial and in disease states.Keywords
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