A specific method for the determination of free acetate in blood and tissues
- 1 August 1961
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 80 (2), 393-397
- https://doi.org/10.1042/bj0800393
Abstract
A specific enzymic method for the quantitative determination of acetate in the range 1-15[mu]g. in a volume not exceeding 3 ml. is described. The procedure involves diffusion in Conway microdiffusion units and reaction with sulphanilamide by means of a pigeon-liver enzyme preparation, which is stable at -20[degree] for at least 1 year. The method is highly specific for acetate. Interference from a number of substances examined is negligible. The accuracy is about 0.3 [mu]g. (S.D.) independent of the concentration in the range covered.Keywords
This publication has 5 references indexed in Scilit:
- The metabolism of acetaldehyde in mammalian tissues. Reactions in rat-liver suspensions under anaerobic conditionsBiochemical Journal, 1959
- ACYL ADENYLATES: THE SYNTHESIS AND PROPERTIES OF ADENYL ACETATEJournal of Biological Chemistry, 1956
- MICRODIFFUSION OF ACETIC ACID AS AN ASSAY FOR ACETYLCHOLINESTERASEJournal of Biological Chemistry, 1955
- A SPECIFIC ENZYMATIC MICROMETHOD FOR THE DETERMINATION OF ACETATEJournal of Biological Chemistry, 1954
- An effect of bicarbonate on the oxidation of pyruvate by kidney homogenatesBiochemical Journal, 1953