Zum Ablauf der Invasion von Virusnucleinsäure in Gewebekulturzelle

Abstract

The adsorption and penetration of polio-virus ribonucleic-acid (RNA) into tissue culture cells can be separated experimentally. The process of adsorption can be interrupted by diluting the RNA at certain times after seeding, whereas the process of penetration can be followed by the application of RNAase. The cells are sensitized for uptake of RNA by hypertonic saline. As long as the cells are in hypertonic saline, the greater part of the RNA remains in a state susceptible to RNAase. The adsorbed RNA enters the cells in an isotonic solution and is no longer sensitive to the action of external RNAase even when it is applied in hypertonic solutions.