Human α1 ‐antitrypsin expression in Xenopus oocytes
- 1 December 1985
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 153 (2), 361-365
- https://doi.org/10.1111/j.1432-1033.1985.tb09311.x
Abstract
Injection of equivalent amounts of normal (PiMM) or abnormal (PiZZ) .alpha.1-antitrypsin mRNA into Xenopus oocytes resulted in secretion of both the normal and abnormal .alpha.1-antitrypsin. A much lower proportion of the abnormal protein was secreted, and Z .alpha.1-antitrypsin that was not secreted accumulated within the cell in a high-mannose form. The time taken for secretion of the normal and abnormal proteins was identical. Both the secreted and intracellular .alpha.1-antitrypsin synthesized by oocytes were functionally active.This publication has 20 references indexed in Scilit:
- α1-Antitrypsin and the serpins: variation and countervariationTrends in Biochemical Sciences, 1985
- α1-Antitrypsin deficiency detection by direct analysis of the mutation in the geneNature, 1983
- Hepatoma secretory proteins migrate from rough endoplasmic reticulum to Golgi at characteristic ratesNature, 1983
- Translation and processing of normal (PiMM) and abnormal (PiZZ) human α1‐antitrypsinFEBS Letters, 1983
- In vitro synthesis of M and Z forms of human α1‐antitrypsinFEBS Letters, 1982
- Liver Disease in Alpha1-Antitrypsin Deficiency Detected by Screening of 200,000 InfantsNew England Journal of Medicine, 1976
- Purification of alpha1-Antitrypsin from Plasma through Thiol-Disulfide InterchangeEuropean Journal of Biochemistry, 1975
- PREPARATION AND ASSESSMENT OF RADIOACTIVE TRACERSBritish Medical Bulletin, 1974
- Isolation of mammalian messenger ribonucleic acidBiochemistry, 1972
- Alpha-1-Antitrypsin DeficiencyHospital Practice, 1971