Benzyloxime Derivatives of Steroids. A New Metabolic Profile Procedure for Human Urinary Steroids Human Urinary Steroids

Abstract

Benzyloxime (BO) and benzyloxime-trimethylsilyl (BO-TMSi) derivatives of steroids may be prepared in the same way as methoxime (MO) and MO-TMSi derivatives. They are eluted much later than the corresponding MO derivatives. This effect makes it easy to distinguish steroids with a reactive ketone group from closely related steroids containing hydroxyl groups alone, or with an unreactive ketone (11-one) group. The mass spectra (electron impact) have characteristic peaks which are readily recognized. These derivatives may be employed in metabolic profile separations of adult and newborn human urinary steroids. A Dexsil 300 column was employed in adult studies; an SE-30 column was used in infant studies.