Sucrose Synthase, a Cytosolic Enzyme in Protoplasts of Jerusalem Artichoke Tubers (Helianthus tuberosus L.)
- 1 October 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 88 (2), 239-241
- https://doi.org/10.1104/pp.88.2.239
Abstract
The exact subcellular location of sucrose synthase (UDP-d-glucose: d-fructose 2-α-d-glucosyltransferase, EC 2.4.1.13) in Helianthus tuberosus tubers was studied by comparison of its activity in protoplasts with that of vacuoles isolated from them. Assuming 100% of the β-N-acetylglucosaminidase activity to be of vacuolar origin, less than 5% of both the sucrose synthase activity and the extravacuolar marker NAD-malate dehydrogenase was detected in the vacuole preparations. Sucrose synthase is therefore an extravacuolar enzyme. Its role in the inulin metabolism of H. tuberosus is discussed.This publication has 9 references indexed in Scilit:
- Fructan and its relationship to abiotic stress tolerance in plantsCellular and Molecular Life Sciences, 2009
- Sucrose Synthase of Soybean NodulesPlant Physiology, 1985
- Localization of Fructan Metabolism in the Vacuoles Isolated from Protoplasts of Jerusalem Artichoke Tubers (Helianthus tuberosus L.)Journal of Plant Physiology, 1984
- Enzymic properties of amyloplasts form suspension cultures of soybeanBiochimica et Biophysica Acta (BBA) - General Subjects, 1983
- Subcellular Distribution of Gluconeogenetic Enzymes in Germinating Castor Bean EndospermPlant Physiology, 1979
- A procedure for the assay of sucrose synthetase and sucrose phosphate synthetase in plant homogenatesAnalytical Biochemistry, 1979
- Sucrose-Uridine Diphosphate Glucosyltransferase from Jerusalem Artichoke TubersJournal of Biological Chemistry, 1964
- The biosynthesis of sucrose.1955