Maintenance of Normal in situ Chromosomal Features in Long-Term Tissue Cultures

Abstract

Clonal isolates from a rapidly proliferating fibroblast-like derivative have retained the classic diploid chromosome relationship over a period of many transplant generations. The readily identified members of the 11 pairs of chromosomes, including the sex chromosomes (X₁ and X₂), have aided in localizing minute structural alterations within recloned diploid and aneuploid sublines.